A Secret Weapon For thr77
A Secret Weapon For thr77
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in several tumors, which results in chromosome instability, and thus may lead to tumorigenesis. This protein was found to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical function in advertising and marketing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already explained. Two additional splice variants had been noted but in depth nucleotide sequence information is not nevertheless available.
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Blocking peptides are peptides that bind precisely to your goal antibody and block antibody binding. These peptide commonly is made up of the epitope recognized by the antibody.
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This guidebook outlines additional probable optimization of mobile and immunoassay parameters to be sure the absolute best results are...
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to ensure the very best results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, you can see which staining is distinct.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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